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干细胞

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    现刊
    Teratoma Formation Assay for Assessing Pluripotency and Tumorigenicity of Pluripotent Stem Cells
    采用畸胎瘤形成分析法评估多能干细胞的多能性和致瘤性
    作者:Shingo Miyawaki, Yohei Okada, Hideyuki Okano and Kyoko Miura日期:08/20/2017,浏览量:79,Q&A: 0
    [Abstract] Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma formation assay is widely used as an index of pluripotency (Evans and Kaufman, 1981; Hentze et al. ...
    Exit from Pluripotency Assay of Mouse Embryonic Stem Cells
    小鼠胚胎干细胞退出多能性测定实验
    作者:Daniel Cirera-Salinas and Constance Ciaudo日期:08/20/2017,浏览量:91,Q&A: 0
    [Abstract] A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed (Ying et al., 2003; Betschinger et al., 2013). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation ...
    A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem Cells
    人骨髓间充质干细胞共培养实验测定生物力学诱导的TNF-α抑制功效
    作者:Miguel F. Diaz, Siobahn M. Evans, Scott D. Olson, Charles S. Cox Jr and Pamela L. Wenzel日期:08/20/2017,浏览量:74,Q&A: 0
    [Abstract] The beneficial effects of mesenchymal stem cell (MSC)-based cellular therapies are believed to be mediated primarily by the ability odansf MSCs to suppress inflammation associated with chronic or acute injury, infection, autoimmunity, and graft-versus-host disease. To specifically address the effects of frictional force caused by blood flow, or ...
    Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue
    从鼠脂肪组织中分离和扩增间充质干细胞
    作者:Natalia V. Andreeva, Alexandra A. Dalina and Alexander V. Belyavsky日期:08/20/2017,浏览量:76,Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical studies of MSC effects in various murine disease models. The appropriate cell preparations with ...
    Primary Culture System for Germ Cells from Caenorhabditis elegans Tumorous Germline Mutants
    秀丽隐杆线虫肿瘤生殖细胞系突变体生殖细胞的原代培养系统
    作者:Alexandra S. Vagasi, Mohammad M. Rahman, Snehal N. Chaudhari and Edward T. Kipreos日期:08/05/2017,浏览量:111,Q&A: 0
    [Abstract] The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous mutants can be fully cellularized and isolated intact from the mutant animals. ...
    Mouse Müller Cell Isolation and Culture
    小鼠Müller细胞分离与培养
    作者:Xiao Liu, Luosheng Tang and Yongqing Liu日期:08/05/2017,浏览量:124,Q&A: 0
    [Abstract] Müller cells are the major supportive and protective glial cells across the retina. Unlike in fish, they have lost the capacity to regenerate the retina in mammals. But, mammalian Müller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a merit in cell-based ...
    Differentiation of Human Induced Pluripotent Stem Cells (iPS Cells) and Embryonic Stem Cells (ES Cells) into Dendritic Cell (DC) Subsets
    诱导的人多能干细胞(iPS细胞)和胚胎干细胞(ES细胞)向树突状细胞(DC)亚型的分化
    作者:Stephanie Sontag, Malrun Förster, Kristin Seré and Martin Zenke日期:08/05/2017,浏览量:130,Q&A: 0
    [Abstract] Induced pluripotent stem cells (iPS cells) are engineered stem cells, which exhibit properties very similar to embryonic stem cells (ES cells; Takahashi and Yamanaka, 2016). Both iPS cells and ES cells have an extraordinary self-renewal capacity and can differentiate into all cell types of our body, including hematopoietic stem/progenitor cells ...
    Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
    将人外周角膜球体植入尸体人角膜组织
    作者:Jeremy John Mathan, Salim Ismail, Jennifer Jane McGhee and Trevor Sherwin日期:07/20/2017,浏览量:222,Q&A: 0
    [Abstract] Stem and progenitor cells isolated from human limbal tissue can be cultured in vitro as spheres. These spheres have potential for use as transplantable elements for the repopulation of corneal tissue (Mathan et al., 2016). Herein we describe the detailed protocol for the implantation of human corneal spheres into cadaveric human ...
    Microvesicle Isolation from Rat Brain Extract Treated Human Mesenchymal Stem Cells
    从大鼠脑提取物处理的人间充质干细胞中分离微泡
    作者:Ji Yong Lee, Seong-Mi Choi and Han-Soo Kim日期:07/05/2017,浏览量:441,Q&A: 0
    [Abstract] Microvesicle (MVs) are submicron-sized membranous vesicles that are either actively released from cells via secretory compartments or shed from cell surface membranes. MVs are generated by many cell types and serve as vehicles that transfer biological information (e.g., protein, mRNA, and miRNA) to distant cells, thereby affecting their ...
    Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth
    甲醛法固定细胞外基质蛋白层以增强原代细胞生长
    作者:Natalia V. Andreeva and Alexander V. Belyavsky日期:07/05/2017,浏览量:405,Q&A: 0
    [Abstract] Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation. However, the effects of such protein layers are usually rather modest, which might be explained by the loss immobilized proteins ...