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细胞生物学

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    现刊
    Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells
    源自小鼠骨髓的骨髓源性抑制细胞的分化及其与脾树突状细胞的共培养
    作者:Giada Mondanelli and Claudia Volpi日期:09/20/2017,浏览量:4,Q&A: 0
    Myeloid-derived suppressor cells (MDSCs) possess the ability to suppress the immune response, and to amplify the regulatory properties of other immune cells, i.e., dendritic cells. Here we describe a protocol in which MDSCs were ...
    Phagocytosis Assay of Necroptotic Cells by Cardiac Myofibroblasts
    心肌成纤维细胞对坏死凋亡细胞的吞噬作用分析
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:4,Q&A: 0
    In myocardial infarction (MI), a plenty of cardiomyocytes undergo necrosis and necroptosis due to the lack of oxygen and nutrients. The dead cardiomyocytes are promptly engulfed by phagocytes. When the dead cells are not engulfed, the noxious ...
    An Assay to Determine Phagocytosis of Apoptotic Cells by Cardiac Macrophages and Cardiac Myofibroblasts
    一种测定心肌巨噬细胞和心肌成纤维细胞对凋亡细胞吞噬作用的方法
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:3,Q&A: 0
    In myocardial infarction (MI), a number of cardiomyocytes undergo apoptosis. These apoptotic cardiomyocytes are promptly engulfed by phagocytes. If the dead cells are not engulfed, their noxious contents are released outside, resulting in induction ...
    Freeze-fracture-etching Electron Microscopy for Facile Analysis of Yeast Ultrastructure
    电镜冷冻断裂蚀刻技术用于酵母超微结构的简易分析
    作者:Takuma Tsuji and Toyoshi Fujimoto日期:09/20/2017,浏览量:8,Q&A: 0
    We describe a streamlined method that enables the quick observation of yeast ultrastructure by electron microscopy (EM). Yeast cells are high-pressure frozen, freeze-fractured to cut across the cytoplasm, and freeze-etched to sublimate ice in the ...
    Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
    胚胎大鼠海马和脑皮质神经元原代培养物的制备
    This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for in vitro experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal ...
    Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells
    用于监测蒽环类药物进入哺乳动物细胞的摄取分析
    作者:Nicolas Brosseau, Emil Andreev and Dindial Ramotar日期:09/20/2017,浏览量:4,Q&A: 0
    Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be ...
    Peroxisome Motility Measurement and Quantification Assay
    过氧化物酶体能动性测量和定量分析
    作者:Jeremy Metz, Inês G. Castro and Michael Schrader日期:09/05/2017,浏览量:85,Q&A: 0
    Organelle movement, distribution and interaction contribute to the organisation of the eukaryotic cell. Peroxisomes are multifunctional organelles which contribute to cellular lipid metabolism and ROS homeostasis. They distribute uniformly in ...
    Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures
    在细胞培养中建立基于多重图像的自噬RNAi筛选方法
    作者:Jennifer Jung and Christian Behrends日期:09/05/2017,浏览量:118,Q&A: 0
    Autophagy is a recycling pathway, in which intracellular cargoes including protein aggregates and bacteria are engulfed by autophagosomes and subsequently degraded after fusion with lysosomes. Dysregulation of this process is involved in several ...
    Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from Tgfbr2-deficient Squamous Cell Carcinoma
    从Tgfbr2缺乏鳞状细胞癌分离上皮CD34+癌干细胞
    作者:Heather A. McCauley and Géraldine Guasch日期:09/05/2017,浏览量:135,Q&A: 0
    Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation ...
    In vivo Priming of T Cells with in vitro Pulsed Dendritic Cells: Popliteal Lymph Node Assay
    使用体外脉冲树突状细胞体外致敏T细胞: 腘窝淋巴结实验
    作者:Songjie Cai, Masayuki Fujino, Lina Lu and Xiao-Kang Li日期:09/05/2017,浏览量:85,Q&A: 0
    One-way mixed lymphocyte reaction (MLR) is a classic tool to measure how T cells react to external stimuli. However, MLR is an in vitro reaction system, which shows different response intensity compared with in vivo trails ...
    Determination of Local pH Differences within Living Salmonella Cells by High-resolution pH Imaging Based on pH-sensitive GFP Derivative, pHluorin(M153R)
    采用基于pH敏感性GFP衍生物pHluorin(M153R)的高分辨率pH成像测定活体沙门氏菌细胞中局部pH值的差异
    作者:Yusuke V. Morimoto, Nobunori Kami-ike, Keiichi Namba and Tohru Minamino日期:09/05/2017,浏览量:64,Q&A: 0
    The bacterial flagellar type III protein export apparatus is composed of a transmembrane export gate complex and a cytoplasmic ATPase complex. The export apparatus requires ATP hydrolysis and the proton motive force across the cytoplasmic membrane ...
    Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells
    利用多色受激发射损耗(STED)显微镜获取呼​​吸道合胞体病毒颗粒和感染细胞的高分辨率图像
    Human respiratory syncytial virus (RSV) infection in human lung epithelial A549 cells induces filopodia, cellular protrusions consisting of F-actin, that extend to neighboring uninfected cells (Mehedi et al., 2016). High-resolution imaging ...
    Isolation and Quantification of Plant Extracellular Vesicles
    植物细胞外囊泡的分离和定量
    作者:Brian D. Rutter, Katie L. Rutter and Roger W. Innes日期:09/05/2017,浏览量:137,Q&A: 0
    Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and ...
    Isolation of Rodent Brain Vessels
    啮齿动物脑血管分离
    作者:Cristina Carvalho and Paula I. Moreira日期:09/05/2017,浏览量:89,Q&A: 0
    The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation ...
    FM1-43 Photoconversion and Electron Microscopy Analysis at the Drosophila Neuromuscular Junction
    果蝇神经肌肉接头处FM1-43光转换和电子显微镜分析
    作者:Nadezhda S. Sabeva and Maria Bykhovskaia日期:09/05/2017,浏览量:130,Q&A: 0
    We developed a protocol for photoconversion of endocytic marker FM1-43 followed by electron microscopy analysis of synaptic boutons at the Drosophila neuromuscular junction. This protocol allows detection of stained synaptic vesicle even ...
    Isolation of Mouse Cardiac Neural Crest Cells and Their Differentiation into Smooth Muscle Cells
    小鼠心脏神经嵴细胞的分离及向平滑肌细胞的分化
    作者:Xia Wang and Sophie Astrof日期:09/05/2017,浏览量:87,Q&A: 0
    Cardiac neural crest cells (CNCCs) originate at the dorsal edge of the neural tube between the otic pit and the caudal edge of the 3rd somite, and migrate into the pharyngeal arches and the heart. We have shown that fibronectin (Fn1) ...
    GFP-Grb2 Translocation Assay Using High-content Imaging to Screen for Modulators of EGFR-signaling
    通过高含量成像观测GFP-Grb2易位筛查EGFR信号转导调控因子
    作者:Julia Petschnigg and Robin Ketteler日期:09/05/2017,浏览量:117,Q&A: 0
    High-content screening is a useful tool to understand complex cellular processes and to identify genes, proteins or small molecule compounds that modulate such pathways. High-content assays monitor the function of a protein or pathway by visualizing ...
    Staining of Membrane Receptors with Fluorescently-labeled DNA Aptamers for Super-resolution Imaging
    用荧光标记的DNA吸附剂染色膜受体以实现超分辨率成像
    作者:Maria Angela Gomes de Castro, Claudia Höbartner and Felipe Opazo日期:09/05/2017,浏览量:88,Q&A: 0
    One of the most prominent applications of fluorescent super-resolution microscopy is the study of nanodomain arrangements of receptors and the endocytic pathway. Staining methods are becoming crucial for answering questions on the nanoscale, ...
    Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells
    源自小鼠骨髓的骨髓源性抑制细胞的分化及其与脾树突状细胞的共培养
    作者:Giada Mondanelli and Claudia Volpi日期:09/20/2017,浏览量:4,Q&A: 0
    [Abstract] Myeloid-derived suppressor cells (MDSCs) possess the ability to suppress the immune response, and to amplify the regulatory properties of other immune cells, i.e., dendritic cells. Here we describe a protocol in which MDSCs were differentiated from murine bone marrow cells, and CD11c+ dendritic cells were purified from murine ...
    Phagocytosis Assay of Necroptotic Cells by Cardiac Myofibroblasts
    心肌成纤维细胞对坏死凋亡细胞的吞噬作用分析
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:4,Q&A: 0
    [Abstract] In myocardial infarction (MI), a plenty of cardiomyocytes undergo necrosis and necroptosis due to the lack of oxygen and nutrients. The dead cardiomyocytes are promptly engulfed by phagocytes. When the dead cells are not engulfed, the noxious contents of the cells are released outside, and thus, induce inflammation, and obstruct the function of ...
    An Assay to Determine Phagocytosis of Apoptotic Cells by Cardiac Macrophages and Cardiac Myofibroblasts
    一种测定心肌巨噬细胞和心肌成纤维细胞对凋亡细胞吞噬作用的方法
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:3,Q&A: 0
    [Abstract] In myocardial infarction (MI), a number of cardiomyocytes undergo apoptosis. These apoptotic cardiomyocytes are promptly engulfed by phagocytes. If the dead cells are not engulfed, their noxious contents are released outside, resulting in induction of inflammation. Therefore, the removal of these dead cells is necessary. However, the contribution ...
    Freeze-fracture-etching Electron Microscopy for Facile Analysis of Yeast Ultrastructure
    电镜冷冻断裂蚀刻技术用于酵母超微结构的简易分析
    作者:Takuma Tsuji and Toyoshi Fujimoto日期:09/20/2017,浏览量:8,Q&A: 0
    [Abstract] We describe a streamlined method that enables the quick observation of yeast ultrastructure by electron microscopy (EM). Yeast cells are high-pressure frozen, freeze-fractured to cut across the cytoplasm, and freeze-etched to sublimate ice in the cytosol and the organelle lumen. The cellular structures delineated by these procedures are coated by ...
    Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
    胚胎大鼠海马和脑皮质神经元原代培养物的制备
    [Abstract] This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for in vitro experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal precursor cells are not yet fully differentiated. This helps increasing the quality and quantity of ...
    Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells
    用于监测蒽环类药物进入哺乳动物细胞的摄取分析
    作者:Nicolas Brosseau, Emil Andreev and Dindial Ramotar日期:09/20/2017,浏览量:4,Q&A: 0
    [Abstract] Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.
    Peroxisome Motility Measurement and Quantification Assay
    过氧化物酶体能动性测量和定量分析
    作者:Jeremy Metz, Inês G. Castro and Michael Schrader日期:09/05/2017,浏览量:85,Q&A: 0
    [Abstract] Organelle movement, distribution and interaction contribute to the organisation of the eukaryotic cell. Peroxisomes are multifunctional organelles which contribute to cellular lipid metabolism and ROS homeostasis. They distribute uniformly in mammalian cells and move along microtubules via kinesin and dynein motors. Their metabolic cooperation ...
    Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures
    在细胞培养中建立基于多重图像的自噬RNAi筛选方法
    作者:Jennifer Jung and Christian Behrends日期:09/05/2017,浏览量:118,Q&A: 0
    [Abstract] Autophagy is a recycling pathway, in which intracellular cargoes including protein aggregates and bacteria are engulfed by autophagosomes and subsequently degraded after fusion with lysosomes. Dysregulation of this process is involved in several human diseases such as cancer or neurodegeneration. Hence, advancing our understanding of how autophagy ...
    Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from Tgfbr2-deficient Squamous Cell Carcinoma
    从Tgfbr2缺乏鳞状细胞癌分离上皮CD34+癌干细胞
    作者:Heather A. McCauley and Géraldine Guasch日期:09/05/2017,浏览量:135,Q&A: 0
    [Abstract] Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation strategies relying on cell surface markers or fluorescent reporters are essential, and the isolation ...
    In vivo Priming of T Cells with in vitro Pulsed Dendritic Cells: Popliteal Lymph Node Assay
    使用体外脉冲树突状细胞体外致敏T细胞: 腘窝淋巴结实验
    作者:Songjie Cai, Masayuki Fujino, Lina Lu and Xiao-Kang Li日期:09/05/2017,浏览量:85,Q&A: 0
    [Abstract] One-way mixed lymphocyte reaction (MLR) is a classic tool to measure how T cells react to external stimuli. However, MLR is an in vitro reaction system, which shows different response intensity compared with in vivo trails sometimes due to the lack of cytokines, tissue matrix and other immune response associated factors. The ...