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植物科学

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    现刊
    Isolation and Detection of the Chlorophyll Catabolite Hydroxylating Activity from Capsicum annuum Chromoplasts
    辣椒青霉色素中叶绿素的分离及含量羟化活性的测定
    作者:Mareike Hauenstein and Stefan Hörtensteiner日期:09/20/2017,浏览量:12,Q&A: 0
    Hydroxylation of chlorophyll catabolites at the so-called C32 position (Hauenstein et al., 2016) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum ...
    Detection of Protein S-nitrosothiols (SNOs) in Plant Samples on Diaminofluorescein (DAF) Gels
    在二氨基荧光素(DAF)凝胶上检测植物样品中的蛋白质S-亚硝基硫醇(SNO)
    作者:Marta Rodríguez-Ruiz, Paulo T. Mioto, José M. Palma and Francisco J. Corpas 日期:09/20/2017,浏览量:10,Q&A: 0
    In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for ...
    Construction of a Single Transcriptional Unit for Expression of Cas9 and Single-guide RNAs for Genome Editing in Plants
    构建共表达Cas9和sgRNAs的单一独立转录单元用于植物基因组编辑
    作者:Xu Tang, Zhaohui Zhong, Xuelian Zheng and Yong Zhang日期:09/05/2017,浏览量:145,Q&A: 0
    The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein9 (Cas9) is a simple and efficient tool for genome editing in many organisms including plant and crop species. The sgRNAs of the CRISPR/Cas9 system are ...
    Isolation and Quantification of Plant Extracellular Vesicles
    植物细胞外囊泡的分离和定量
    作者:Brian D. Rutter, Katie L. Rutter and Roger W. Innes日期:09/05/2017,浏览量:137,Q&A: 0
    Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and ...
    Ubiquitin Proteasome Activity Measurement in Total Plant Extracts
    植物总提取物中泛素蛋白酶体活性测定
    作者:Suayib Üstün and Frederik Börnke日期:09/05/2017,浏览量:144,Q&A: 0
    The fine-tuned balance of protein level, conformation and location within the cell is vital for the dynamic changes required for a cell to respond to a given stimulus. This requires the regulated turnover of damaged or short-lived proteins through ...
    Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella
    拟小球藻中总磷酸盐和多磷酸盐的提取和钼蓝法定量
    作者:Shuhei Ota and Shigeyuki Kawano日期:09/05/2017,浏览量:73,Q&A: 0
    Inorganic phosphorus is a non-renewable resource and an essential element for life on Earth. Organisms such as algae, protists, and animals can store phosphate (Pi) through uptake of Pi as polyphosphate (poly-P), which is a linear polymer of ...
    Isolation and Detection of the Chlorophyll Catabolite Hydroxylating Activity from Capsicum annuum Chromoplasts
    辣椒青霉色素中叶绿素的分离及含量羟化活性的测定
    作者:Mareike Hauenstein and Stefan Hörtensteiner日期:09/20/2017,浏览量:12,Q&A: 0
    [Abstract] Hydroxylation of chlorophyll catabolites at the so-called C32 position (Hauenstein et al., 2016) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum annuum chromoplast membranes as a source of the hydroxylating activity, which converts the ...
    Detection of Protein S-nitrosothiols (SNOs) in Plant Samples on Diaminofluorescein (DAF) Gels
    在二氨基荧光素(DAF)凝胶上检测植物样品中的蛋白质S-亚硝基硫醇(SNO)
    作者:Marta Rodríguez-Ruiz, Paulo T. Mioto, José M. Palma and Francisco J. Corpas 日期:09/20/2017,浏览量:10,Q&A: 0
    [Abstract] In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (King et al., 2005) for plant samples. Briefly, ...
    Construction of a Single Transcriptional Unit for Expression of Cas9 and Single-guide RNAs for Genome Editing in Plants
    构建共表达Cas9和sgRNAs的单一独立转录单元用于植物基因组编辑
    作者:Xu Tang, Zhaohui Zhong, Xuelian Zheng and Yong Zhang日期:09/05/2017,浏览量:145,Q&A: 0
    [Abstract] The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein9 (Cas9) is a simple and efficient tool for genome editing in many organisms including plant and crop species. The sgRNAs of the CRISPR/Cas9 system are typically expressed from RNA polymerase III promoters, such as U6 and U3. In many transformation events, ...
    Isolation and Quantification of Plant Extracellular Vesicles
    植物细胞外囊泡的分离和定量
    作者:Brian D. Rutter, Katie L. Rutter and Roger W. Innes日期:09/05/2017,浏览量:137,Q&A: 0
    [Abstract] Extracellular vesicles (EVs) play an important role in intercellular communication by transporting proteins and RNA. While plant cells secrete EVs, they have only recently been isolated and questions regarding their biogenesis, release, uptake and function remain unanswered. Here, we present a detailed protocol for isolating EVs from the ...
    Ubiquitin Proteasome Activity Measurement in Total Plant Extracts
    植物总提取物中泛素蛋白酶体活性测定
    作者:Suayib Üstün and Frederik Börnke日期:09/05/2017,浏览量:144,Q&A: 0
    [Abstract] The fine-tuned balance of protein level, conformation and location within the cell is vital for the dynamic changes required for a cell to respond to a given stimulus. This requires the regulated turnover of damaged or short-lived proteins through the ubiquitin proteasome system (UPS). Thus, the protease activity of the proteasome is adjusted to ...
    Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella
    拟小球藻中总磷酸盐和多磷酸盐的提取和钼蓝法定量
    作者:Shuhei Ota and Shigeyuki Kawano日期:09/05/2017,浏览量:73,Q&A: 0
    [Abstract] Inorganic phosphorus is a non-renewable resource and an essential element for life on Earth. Organisms such as algae, protists, and animals can store phosphate (Pi) through uptake of Pi as polyphosphate (poly-P), which is a linear polymer of orthophosphate residues linked by high-energy phosphoanhydride bonds. Here, we describe procedures for ...
    [Bio101] Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
    用于观察叶面气孔和其他细胞的叶片透化方案
    作者:Nidhi Sharma日期:09/05/2017,浏览量:74,Q&A: 0
    [Abstract] In this protocol, leaves are cleared and fixed in an ethanol and acetic acid solution, and mounted in Hoyer’s solution. The cleared leaves are imaged under differential interference contrast (DIC) microscope. This protocol is beneficial for studying stomata, hair cells, and other epidermal cells in plants.
    TUNEL Assay to Assess Extent of DNA Fragmentation and Programmed Cell Death in Root Cells under Various Stress Conditions
    各种胁迫条件下根细胞DNA片段化和程序性细胞死亡的TUNEL法评估
    作者:Amit K. Tripathi, Ashwani Pareek and Sneh Lata Singla-Pareek日期:08/20/2017,浏览量:84,Q&A: 0
    [Abstract] DNA damage is one of the common consequences of exposure to various stress conditions. Different methods have been developed to accurately assess DNA damage and fragmentation in cells and tissues exposed to different stress agents. However, owing to the presence of firm cellulosic cell wall and phenolics, plant cells and tissues are not easily ...
    Using Silicon Polymer Impression Technique and Scanning Electron Microscopy to Measure Stomatal Aperture, Morphology, and Density
    使用硅聚合物印模技术和扫描电子显微镜技术测量气孔的开度、形态和密度
    作者:Hui-Chen Wu, Ya-Chen Huang, Chia-Hung Liu and Tsung-Luo Jinn日期:08/20/2017,浏览量:73,Q&A: 0
    [Abstract] The number of stomata on leaves can be affected by intrinsic development programming and various environmental factors, in addition the control of stomatal apertures is extremely important for the plant stress response. In response to elevated temperatures, transpiration occurs through the stomatal apertures, allowing the leaf to cool through ...
    Quantification of Membrane Damage/Cell Death Using Evan’s Blue Staining Technique
    采用伊文思蓝染色技术定量膜损伤/细胞死亡
    [Abstract] Membrane damage is a hallmark of both biotic and abiotic stress responses. The membrane determines the ability of a cell to sustain altered environmental conditions and hence can be used as a biomarker to assess stress-induced cell damage or death. We present an easy, quick, cost-effective, staining and spectrophotometric method to assess membrane ...