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癌症生物学

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    现刊
    Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells
    用于监测蒽环类药物进入哺乳动物细胞的摄取分析
    作者:Nicolas Brosseau, Emil Andreev and Dindial Ramotar日期:09/20/2017,浏览量:4,Q&A: 0
    Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be ...
    Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures
    在细胞培养中建立基于多重图像的自噬RNAi筛选方法
    作者:Jennifer Jung and Christian Behrends日期:09/05/2017,浏览量:118,Q&A: 0
    Autophagy is a recycling pathway, in which intracellular cargoes including protein aggregates and bacteria are engulfed by autophagosomes and subsequently degraded after fusion with lysosomes. Dysregulation of this process is involved in several ...
    Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from Tgfbr2-deficient Squamous Cell Carcinoma
    从Tgfbr2缺乏鳞状细胞癌分离上皮CD34+癌干细胞
    作者:Heather A. McCauley and Géraldine Guasch日期:09/05/2017,浏览量:135,Q&A: 0
    Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation ...
    GFP-Grb2 Translocation Assay Using High-content Imaging to Screen for Modulators of EGFR-signaling
    通过高含量成像观测GFP-Grb2易位筛查EGFR信号转导调控因子
    作者:Julia Petschnigg and Robin Ketteler日期:09/05/2017,浏览量:117,Q&A: 0
    High-content screening is a useful tool to understand complex cellular processes and to identify genes, proteins or small molecule compounds that modulate such pathways. High-content assays monitor the function of a protein or pathway by visualizing ...
    Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells
    用于监测蒽环类药物进入哺乳动物细胞的摄取分析
    作者:Nicolas Brosseau, Emil Andreev and Dindial Ramotar日期:09/20/2017,浏览量:4,Q&A: 0
    [Abstract] Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.
    Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures
    在细胞培养中建立基于多重图像的自噬RNAi筛选方法
    作者:Jennifer Jung and Christian Behrends日期:09/05/2017,浏览量:118,Q&A: 0
    [Abstract] Autophagy is a recycling pathway, in which intracellular cargoes including protein aggregates and bacteria are engulfed by autophagosomes and subsequently degraded after fusion with lysosomes. Dysregulation of this process is involved in several human diseases such as cancer or neurodegeneration. Hence, advancing our understanding of how autophagy ...
    Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from Tgfbr2-deficient Squamous Cell Carcinoma
    从Tgfbr2缺乏鳞状细胞癌分离上皮CD34+癌干细胞
    作者:Heather A. McCauley and Géraldine Guasch日期:09/05/2017,浏览量:135,Q&A: 0
    [Abstract] Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation strategies relying on cell surface markers or fluorescent reporters are essential, and the isolation ...
    GFP-Grb2 Translocation Assay Using High-content Imaging to Screen for Modulators of EGFR-signaling
    通过高含量成像观测GFP-Grb2易位筛查EGFR信号转导调控因子
    作者:Julia Petschnigg and Robin Ketteler日期:09/05/2017,浏览量:117,Q&A: 0
    [Abstract] High-content screening is a useful tool to understand complex cellular processes and to identify genes, proteins or small molecule compounds that modulate such pathways. High-content assays monitor the function of a protein or pathway by visualizing a change in an image-based readout, such as a change in the localization of a reporter protein. ...
    [Bio101] Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
    纯化正确折叠的富含半胱氨酸、包含锌指结构重组蛋白用于结构药物靶向研究:以p300的CH1结构域为例
    作者:Yong Joon Kim, Stefan Kaluz, Anil Mehta, Emily Weinert, Shannon Rivera and Erwin G. Van Meir日期:09/05/2017,浏览量:95,Q&A: 0
    [Abstract] The transcription factor Hypoxia-Inducible Factor (HIF) complexes with the coactivator p300, activating the hypoxia response pathway and allowing tumors to grow. The CH1 and CAD domains of each respective protein form the interface between p300 and HIF. Small molecule compounds are in development that target and inhibit HIF/p300 complex formation, ...
    Teratoma Formation Assay for Assessing Pluripotency and Tumorigenicity of Pluripotent Stem Cells
    采用畸胎瘤形成分析法评估多能干细胞的多能性和致瘤性
    作者:Shingo Miyawaki, Yohei Okada, Hideyuki Okano and Kyoko Miura日期:08/20/2017,浏览量:79,Q&A: 0
    [Abstract] Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma formation assay is widely used as an index of pluripotency (Evans and Kaufman, 1981; Hentze et al. ...
    Rapid Profiling Cell Cycle by Flow Cytometry Using Concurrent Staining of DNA and Mitotic Markers
    DNA和有丝分类marker同步染色后利用流式细胞术快速分析细胞周期
    作者:Yuqing Shen, Paolo Vignali and Ruoning Wang日期:08/20/2017,浏览量:75,Q&A: 0
    [Abstract] The flow cytometric quantitation of DNA content by DNA-binding fluorochrome, propidium iodide (PI) is the most widely used method for cell cycle analysis. However, the commonly used methods are time-consuming and labor-intensive and are incompatible with staining of mitotic markers by fluorescent-labeled antibodies. Here, we report an optimized ...
    Mouse Model of Dextran Sodium Sulfate (DSS)-induced Colitis
    葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠模型
    作者:Srustidhar Das, Surinder K. Batra and Satyanarayana Rachagani日期:08/20/2017,浏览量:66,Q&A: 0
    [Abstract] Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the intestinal tract and is primarily comprised of Crohn’s disease (CD) and ulcerative colitis (UC). Several murine models that include both chemical induced and genetic models have been developed that mimic some aspects of either CD or UC. These models have been instrumental in ...
    Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids
    乳腺肿瘤类器官中反义寡核苷酸介导的基因敲低技术
    作者:Sarah D. Diermeier and David L. Spector日期:08/20/2017,浏览量:77,Q&A: 0
    [Abstract] Primary mammary tumor organoids grown in 3D are an excellent system to study tumor biology. They resemble the organization and physiology of native epithelia more closely than cancer cell lines grown in 2D, and additionally model interactions with the ECM (Boj et al., 2015; Clevers, 2016; Shamir and Ewald, 2014). Mammary tumor organoids ...
    RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells
    RNA干扰筛查鉴定乳腺癌细胞增殖的决定因素
    [Abstract] RNAi screening technology has revealed unknown determinants of various biological signaling pathways in biomedical studies. This protocol provided detailed information about how to use RNAi screening to identify proliferation determinants in breast tumor cells. siRNA-based libraries targeting against Estrogen receptor (ER)-network, including 631 ...