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微生物学

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    现刊
    Drosophila Fecal Sampling
    果蝇粪便取样
    作者:Christine Fink , Jakob von Frieling, Mirjam Knop and Thomas Roeder日期:09/20/2017,浏览量:5,Q&A: 0
    Fecal sampling is a non-invasive method which raises the possibility to study the development and the changes in the microbial community throughout different time points of a fly population or throughout different treatments. This method allows ...
    Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
    酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法
    作者:Rachael M. Giersch and Gregory C. Finnigan日期:09/20/2017,浏览量:4,Q&A: 0
    Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
    Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
    在Sf9昆虫细胞中表达纯化哺乳动物P2X7受体蛋白
    作者:Akira Karasawa and Toshimitsu Kawate日期:09/05/2017,浏览量:143,Q&A: 0
    The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
    Determination of Local pH Differences within Living Salmonella Cells by High-resolution pH Imaging Based on pH-sensitive GFP Derivative, pHluorin(M153R)
    采用基于pH敏感性GFP衍生物pHluorin(M153R)的高分辨率pH成像测定活体沙门氏菌细胞中局部pH值的差异
    作者:Yusuke V. Morimoto, Nobunori Kami-ike, Keiichi Namba and Tohru Minamino日期:09/05/2017,浏览量:64,Q&A: 0
    The bacterial flagellar type III protein export apparatus is composed of a transmembrane export gate complex and a cytoplasmic ATPase complex. The export apparatus requires ATP hydrolysis and the proton motive force across the cytoplasmic membrane ...
    Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells
    利用多色受激发射损耗(STED)显微镜获取呼​​吸道合胞体病毒颗粒和感染细胞的高分辨率图像
    Human respiratory syncytial virus (RSV) infection in human lung epithelial A549 cells induces filopodia, cellular protrusions consisting of F-actin, that extend to neighboring uninfected cells (Mehedi et al., 2016). High-resolution imaging ...
    Rearing of Culex spp. and Aedes spp. Mosquitoes
    库蚊和伊蚊的饲养
    作者:Elizabeth Kauffman, Anne Payne, Mary A. Franke, Michael A. Schmid, Eva Harris and Laura D. Kramer日期:09/05/2017,浏览量:98,Q&A: 0
    Mosquito-transmitted pathogens cause major public health problems and contribute substantially to the global burden of disease. Aedes mosquitoes transmit dengue, Zika, yellow fever, and Chikungunya viruses; Culex mosquitoes ...
    Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2’,7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA)
    使用氧化物探针2',7'-二氯二氟荧光素二乙酸酯(DCFH-DA)检测蓝细菌中活性氧物质(ROS)
    作者:Rajneesh, Jainendra Pathak, Ananya Chatterjee, Shailendra P. Singh and Rajeshwar P. Sinha日期:09/05/2017,浏览量:111,Q&A: 0
    Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells ...
    Sterol Analysis in Kluyveromyces lactis
    乳酸克鲁维酵母中的甾醇分析
    作者:Yvetta Gbelska, Nora Toth Hervay, Marcela Morvova and Alexandra Konecna日期:09/05/2017,浏览量:78,Q&A: 0
    Sterols are essential lipids of most eukaryotic cells with multiple functions (structural, regulatory and developmental). Sterol profile of yeast cells is often determined during the studies of ergosterol synthesis mutants used to uncover a number ...
    Drosophila Fecal Sampling
    果蝇粪便取样
    作者:Christine Fink , Jakob von Frieling, Mirjam Knop and Thomas Roeder日期:09/20/2017,浏览量:5,Q&A: 0
    [Abstract] Fecal sampling is a non-invasive method which raises the possibility to study the development and the changes in the microbial community throughout different time points of a fly population or throughout different treatments. This method allows precise manipulation to trigger the fly’s physiology by nutritional interventions, bacterial infections ...
    Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
    酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法
    作者:Rachael M. Giersch and Gregory C. Finnigan日期:09/20/2017,浏览量:4,Q&A: 0
    [Abstract] Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that recruits the complex to a corresponding genomic target sequence where it induces a double stranded ...
    Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
    在Sf9昆虫细胞中表达纯化哺乳动物P2X7受体蛋白
    作者:Akira Karasawa and Toshimitsu Kawate日期:09/05/2017,浏览量:143,Q&A: 0
    [Abstract] The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been implicated in devastating diseases like chronic pain (North and Jarvis, 2013). However, mechanisms ...
    Determination of Local pH Differences within Living Salmonella Cells by High-resolution pH Imaging Based on pH-sensitive GFP Derivative, pHluorin(M153R)
    采用基于pH敏感性GFP衍生物pHluorin(M153R)的高分辨率pH成像测定活体沙门氏菌细胞中局部pH值的差异
    作者:Yusuke V. Morimoto, Nobunori Kami-ike, Keiichi Namba and Tohru Minamino日期:09/05/2017,浏览量:64,Q&A: 0
    [Abstract] The bacterial flagellar type III protein export apparatus is composed of a transmembrane export gate complex and a cytoplasmic ATPase complex. The export apparatus requires ATP hydrolysis and the proton motive force across the cytoplasmic membrane to unfold and transport flagellar component proteins for the construction of the bacterial flagellum ...
    Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells
    利用多色受激发射损耗(STED)显微镜获取呼​​吸道合胞体病毒颗粒和感染细胞的高分辨率图像
    [Abstract] Human respiratory syncytial virus (RSV) infection in human lung epithelial A549 cells induces filopodia, cellular protrusions consisting of F-actin, that extend to neighboring uninfected cells (Mehedi et al., 2016). High-resolution imaging via stimulated emission depletion (STED) microscopy revealed filamentous RSV particles along these ...
    Rearing of Culex spp. and Aedes spp. Mosquitoes
    库蚊和伊蚊的饲养
    作者:Elizabeth Kauffman, Anne Payne, Mary A. Franke, Michael A. Schmid, Eva Harris and Laura D. Kramer日期:09/05/2017,浏览量:98,Q&A: 0
    [Abstract] Mosquito-transmitted pathogens cause major public health problems and contribute substantially to the global burden of disease. Aedes mosquitoes transmit dengue, Zika, yellow fever, and Chikungunya viruses; Culex mosquitoes transmit West Nile, Japanese encephalitis, and Saint Louis encephalitis viruses, among others. Experiments ...
    Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2’,7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA)
    使用氧化物探针2',7'-二氯二氟荧光素二乙酸酯(DCFH-DA)检测蓝细菌中活性氧物质(ROS)
    作者:Rajneesh, Jainendra Pathak, Ananya Chatterjee, Shailendra P. Singh and Rajeshwar P. Sinha日期:09/05/2017,浏览量:111,Q&A: 0
    [Abstract] Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells that become toxic for the cell survival. Here we present the method for in vivo detection of ...
    Sterol Analysis in Kluyveromyces lactis
    乳酸克鲁维酵母中的甾醇分析
    作者:Yvetta Gbelska, Nora Toth Hervay, Marcela Morvova and Alexandra Konecna日期:09/05/2017,浏览量:78,Q&A: 0
    [Abstract] Sterols are essential lipids of most eukaryotic cells with multiple functions (structural, regulatory and developmental). Sterol profile of yeast cells is often determined during the studies of ergosterol synthesis mutants used to uncover a number of functions for various sterols in yeast cells. Molecular studies of ergosterol biosynthesis have ...
    An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
    利用PEI优化生产,滴定和中和SARS-CoV spike萤光素酶假病毒的方法
    作者:George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton日期:08/20/2017,浏览量:58,Q&A: 0
    [Abstract] The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection reagent PEI. This protocol is optimized for transfection in 6-well plates; however it can be readily ...
    Snapshots of the Signaling Complex DesK:DesR in Different Functional States Using Rational Mutagenesis and X-ray Crystallography
    利用合理化诱变和X射线晶体学分析在不同功能状态下的信号复合体DesK:DesR
    [Abstract] We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli, ensuring an excess of the regulator, was essential for soluble production of the DesK:DesR ...