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    Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells
    源自小鼠骨髓的骨髓源性抑制细胞的分化及其与脾树突状细胞的共培养
    作者:Giada Mondanelli and Claudia Volpi日期:09/20/2017,浏览量:4,Q&A: 0
    Myeloid-derived suppressor cells (MDSCs) possess the ability to suppress the immune response, and to amplify the regulatory properties of other immune cells, i.e., dendritic cells. Here we describe a protocol in which MDSCs were ...
    Phagocytosis Assay of Necroptotic Cells by Cardiac Myofibroblasts
    心肌成纤维细胞对坏死凋亡细胞的吞噬作用分析
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:4,Q&A: 0
    In myocardial infarction (MI), a plenty of cardiomyocytes undergo necrosis and necroptosis due to the lack of oxygen and nutrients. The dead cardiomyocytes are promptly engulfed by phagocytes. When the dead cells are not engulfed, the noxious ...
    An Assay to Determine Phagocytosis of Apoptotic Cells by Cardiac Macrophages and Cardiac Myofibroblasts
    一种测定心肌巨噬细胞和心肌成纤维细胞对凋亡细胞吞噬作用的方法
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:3,Q&A: 0
    In myocardial infarction (MI), a number of cardiomyocytes undergo apoptosis. These apoptotic cardiomyocytes are promptly engulfed by phagocytes. If the dead cells are not engulfed, their noxious contents are released outside, resulting in induction ...
    In vivo Priming of T Cells with in vitro Pulsed Dendritic Cells: Popliteal Lymph Node Assay
    使用体外脉冲树突状细胞体外致敏T细胞: 腘窝淋巴结实验
    作者:Songjie Cai, Masayuki Fujino, Lina Lu and Xiao-Kang Li日期:09/05/2017,浏览量:85,Q&A: 0
    One-way mixed lymphocyte reaction (MLR) is a classic tool to measure how T cells react to external stimuli. However, MLR is an in vitro reaction system, which shows different response intensity compared with in vivo trails ...
    Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells
    源自小鼠骨髓的骨髓源性抑制细胞的分化及其与脾树突状细胞的共培养
    作者:Giada Mondanelli and Claudia Volpi日期:09/20/2017,浏览量:4,Q&A: 0
    [Abstract] Myeloid-derived suppressor cells (MDSCs) possess the ability to suppress the immune response, and to amplify the regulatory properties of other immune cells, i.e., dendritic cells. Here we describe a protocol in which MDSCs were differentiated from murine bone marrow cells, and CD11c+ dendritic cells were purified from murine ...
    Phagocytosis Assay of Necroptotic Cells by Cardiac Myofibroblasts
    心肌成纤维细胞对坏死凋亡细胞的吞噬作用分析
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:4,Q&A: 0
    [Abstract] In myocardial infarction (MI), a plenty of cardiomyocytes undergo necrosis and necroptosis due to the lack of oxygen and nutrients. The dead cardiomyocytes are promptly engulfed by phagocytes. When the dead cells are not engulfed, the noxious contents of the cells are released outside, and thus, induce inflammation, and obstruct the function of ...
    An Assay to Determine Phagocytosis of Apoptotic Cells by Cardiac Macrophages and Cardiac Myofibroblasts
    一种测定心肌巨噬细胞和心肌成纤维细胞对凋亡细胞吞噬作用的方法
    作者:Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya日期:09/20/2017,浏览量:3,Q&A: 0
    [Abstract] In myocardial infarction (MI), a number of cardiomyocytes undergo apoptosis. These apoptotic cardiomyocytes are promptly engulfed by phagocytes. If the dead cells are not engulfed, their noxious contents are released outside, resulting in induction of inflammation. Therefore, the removal of these dead cells is necessary. However, the contribution ...
    In vivo Priming of T Cells with in vitro Pulsed Dendritic Cells: Popliteal Lymph Node Assay
    使用体外脉冲树突状细胞体外致敏T细胞: 腘窝淋巴结实验
    作者:Songjie Cai, Masayuki Fujino, Lina Lu and Xiao-Kang Li日期:09/05/2017,浏览量:85,Q&A: 0
    [Abstract] One-way mixed lymphocyte reaction (MLR) is a classic tool to measure how T cells react to external stimuli. However, MLR is an in vitro reaction system, which shows different response intensity compared with in vivo trails sometimes due to the lack of cytokines, tissue matrix and other immune response associated factors. The ...
    Macrophage Survival Assay Using High Content Microscopy
    使用高内涵显微技术进行巨噬细胞的生存分析
    作者:Remzi Onur Eren and Nicolas Fasel日期:08/20/2017,浏览量:74,Q&A: 0
    [Abstract] Macrophages maintain tissue homoeostasis by regulating inflammation and tissue repair mechanisms. Thus, the fate of macrophages has an impact on the state of the tissue. The aim of this protocol is to quantify macrophage survival using high content microscopy and image processing software. Here, we describe a high-content image based protocol to ...
    Isolation and Analysis of Stromal Vascular Cells from Visceral Adipose Tissue
    .内脏脂肪组织来源基质血管细胞的分离和分析
    作者:Jessica Vu and Wei Ying日期:08/20/2017,浏览量:77,Q&A: 0
    [Abstract] The obesity epidemic is the underlying driver of the type 2 diabetes mellitus epidemic. A remarkable accumulation of various pro-inflammatory immune cells in adipose tissues is a hallmark of obesity and leads to pathogenesis of tissue inflammation and insulin resistance. Here, we describe a detailed protocol to isolate adipose tissue stromal ...
    Establishment of a Human Cell Line Persistently Infected with Sendai Virus
    持续感染仙台病毒的人细胞系的建立
    作者:Christopher Coakley, Cara Peter, Stephanie Fabry and Saurabh Chattopadhyay日期:08/20/2017,浏览量:75,Q&A: 0
    [Abstract] Interferon regulatory transcription factor 3 (IRF3) is a transcription factor that upon activation by virus infection promotes the synthesis of antiviral genes, such as the interferons (Hiscott, 2007). In addition to inducing genes, IRF3 triggers antiviral apoptosis by RIG-I-like receptor-induced IRF3 mediated pathway of apoptosis (RIPA), which is ...
    Bioelectrospray Methodology for Dissection of the Host-pathogen Interaction in Human Tuberculosis
    生物电喷雾法剖析人类结核病中宿主与病原体的相互作用
    作者:Liku B Tezera, Magdalena K Bielecka and Paul T Elkington日期:07/20/2017,浏览量:253,Q&A: 0
    [Abstract] Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where pathology occurs in vivo. To truthfully represent this, an emerging paradigm in biology is the ...
    Preparation of Mosquito Salivary Gland Extract and Intradermal Inoculation of Mice
    蚊子唾液腺提取物的制备与小鼠真皮内接种
    作者:Michael A. Schmid, Elizabeth Kauffman, Anne Payne, Eva Harris and Laura D. Kramer日期:07/20/2017,浏览量:305,Q&A: 0
    [Abstract] Mosquito-transmitted pathogens are among the leading causes of severe disease and death in humans. Components within the saliva of mosquito vectors facilitate blood feeding, modulate host responses, and allow efficient transmission of pathogens, such as Dengue, Zika, yellow fever, West Nile, Japanese encephalitis, and chikungunya viruses, as well ...
    Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
    电泳和组织学检查分析小鼠肠黏蛋白的性质
    作者:Ran Wang and Sumaira Z. Hasnain日期:07/20/2017,浏览量:274,Q&A: 0
    [Abstract] Specialized secretory cells known as goblet cells in the intestine and respiratory epithelium are responsible for the secretion of mucins. Mucins are large heavily glycosylated proteins and typically have a molecular mass higher than 106 Da. These large proteins are densely substituted with short glycan chains, which have many important ...